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Biochemical studies of some Natural antioxidant compounds as free radical scavengers invitro [electronic resource].

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Osman,M.E.A

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Language: English Summary language: Arabic Description: p.233-262Other title:

دراسات بيوكيميائية لبعض المركبات الطبيعية المضادة للاكسدة ككاسحات للشقوق الحرة [Portion of title]

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Journal of biological chemistry and environmental sciences, 2007 v. 2 (1) Part II [electronic resource].

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Journal of Biological Chemistry and Environmental Sciences 2007.v.2(1)IISummary: The antioxidant activity of different plant extracts (Parsley, Turmeric, Eucalyptus, Cumin, Basil, Olive, Lemon balm, Sage, Wild chicory, Rosemary, Lemon) were studied using in vitro and in vivo models. The ethanolic extracts of these plants were examined by DPPH "1,1- diphenyl-2-picryl hydrazyl method for evaluation the antioxidant activity of the different extracts using rutin and L-ascorbic acid as standard antioxidant agents. Also, the peroxide number and the acid value were determined for all plant ethanolic extracts to lookfore the antioxidant activity of each plant extract. In vivo experiment for assessment the antioxidant effect for each plant extract on Fe++IAscorbate model system by using (TBARS) thiobarbituric acid reactive substances method in rat liver mitochondria was performed. The results revealed that the values of DPPH experiment were found to be less or comparable to those of ascorbic acid and rutin, the standard agents. The peroxide number increased daily up to 20 days comparied by butylated hydroxyl toluene (BHT) as standard. Petroselinum sativum extracts by three concentrations "0.02, 0.04, 0.06 g1100g oil" exerted a near values as BHT "46.6, 45.97, 42.8" respectively. Also, Saliva and Curcuma showed a near values "43.27, 44.88" Meq./kg oil after 20 days of the experiment. The other extracts reached high values after 2 weeks. The acid values were increased in all different plant extracts according to the time intervals. The experiment of TBARS of each extract with rat liver mitochondria exerts a significant antioxidant nature with different activity, the highest one was Petroselinum sativum followed by Saliva extracts.

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The antioxidant activity of different plant extracts (Parsley, Turmeric, Eucalyptus, Cumin, Basil, Olive, Lemon balm, Sage, Wild chicory, Rosemary, Lemon) were studied using in vitro and in vivo models. The ethanolic extracts of these plants were examined by DPPH "1,1- diphenyl-2-picryl hydrazyl method for evaluation the antioxidant activity of the different extracts using rutin and L-ascorbic acid as standard antioxidant agents. Also, the peroxide number and the acid value were determined for all plant ethanolic extracts to lookfore the antioxidant activity of each plant extract. In vivo experiment for assessment the antioxidant effect for each plant extract on Fe++IAscorbate model system by using (TBARS) thiobarbituric acid reactive substances method in rat liver mitochondria was performed. The results revealed that the values of DPPH experiment were found to be less or comparable to those of ascorbic acid and rutin, the standard agents. The peroxide number increased daily up to 20 days comparied by butylated hydroxyl toluene (BHT) as standard. Petroselinum sativum extracts by three concentrations "0.02, 0.04, 0.06 g1100g oil" exerted a near values as BHT "46.6, 45.97, 42.8" respectively. Also, Saliva and Curcuma showed a near values "43.27, 44.88" Meq./kg oil after 20 days of the experiment. The other extracts reached high values after 2 weeks. The acid values were increased in all different plant extracts according to the time intervals. The experiment of TBARS of each extract with rat liver mitochondria exerts a significant antioxidant nature with different activity, the highest one was Petroselinum sativum followed by Saliva extracts.

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