Micro and macro genetic damage induced by the insecticide Match in mice genome [electronic resource].

Includes references.

This work aims to disclosing the capability of the well known insecticide Match N-[2,5- dichloro-4-(1,1,2,3,3,3,-hexafluoro-propoxy)-(phenyl)amino]carbonyl]-2,6-difluorobenzamide (CA) that is widely used for controlling certain fruit and leaf caterpillars in inducing micro and macro DNA damage. To investigate its possible genotoxic effects on mammals, different short term genotoxic bioassays were selected and employed. Micro DNA damage was assayed by dominant lethals assay. Macro DNA damage was evaluated by mice bone marrow chromosomes. The effect of Match upon gene expression of serum protein, development and differentiation of sperms was precisely investigated. The frequency of dominant lethal mutations in female mice sired by males treated by Match was significantly higher than that of the control group. There was also a reduction of fertile matings in females mated 15 and 21 days after treatment of males with Match. Analysis of mice bone-marrow chromosomes showed that Match proved to be a positive clastogenic agent, since significant increases of different types of aberrations (e.g., deletions, fragments, Robertsonian centric fusion) were observed and this result was confirmed by the analysis of micronucleated polychromatic erythrocytes.

Summary in Arabic.

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