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Detection of RAPD markers flanking the leaf rust resistance gene,Lr34, in wheat, using bulk segregant analysis [electronic resource].

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Barakat, M. N

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Language: English Summary language: Arabic Description: p. 27-33Other title:

الكشف عن دلائل RAPD المطوقة لجين المقاوم لصدأ الأوراق Lr34 فى القمح باستخدام تحليل BSA (تحليل الانعزالات بطريقة التجميع) [Added title page title]

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Alexandria journal of agricultural research, 2008 v. 53 (1) [electronic resource].

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In: Alexandria journal of agricultural research 2008 v. 53 (1)Summary: Leaf rust, caused by Pllccinia triticina. is an importDnt disease of bread wheat (Triticum aestivrun L) in several production areas ofthe world. The mast effective and economical approach forcontrolling leaf rust disease is to use resistant cultivars. The present objectives were to map the resistance gene, Lr34. in the breeding materials and develop RAPD- (PCR)based markers for marker-auisted selection (MAS).RAPD-PCR(polymerase chain reaction) analysis was conducted, using bulked segregant analysis (BSA ) in a mapping population of sixty F2 individuals derived from a cross between the susceptible cultivar. Salkha 69, and the resistant monogenic line, Lr34. After performing RAPD-PCR analysis with twenty arbitrary lo-mer primers and agarose-gel electrophoruis,mapping of two markers flanking Lr34 Was reported. The closest marker was Pr2 (5'AGGTACCGG3,) at the 2.8 cM with logarithm of the odds to the base 10 (LOD) scores of 15.1. and the other one Pr1(5'GACCGCITGT3, was at 16.3 cM with WD scores of 6.5. Bulked segregant analysis with RAPD and linkage mapping might facilitate selection and enable gene pyramiding for leaf rust resistance in wheat breeding programs.

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Leaf rust, caused by Pllccinia triticina. is an importDnt disease of bread wheat (Triticum aestivrun L) in several production areas ofthe world. The mast effective and economical approach forcontrolling leaf rust disease is to use resistant cultivars. The present objectives were to map the resistance gene, Lr34. in the breeding materials and develop RAPD- (PCR)based markers for marker-auisted selection (MAS).RAPD-PCR(polymerase chain reaction) analysis was conducted, using bulked segregant analysis (BSA ) in a mapping population of sixty F2 individuals derived from a cross between the susceptible cultivar. Salkha 69, and the resistant monogenic line, Lr34. After performing RAPD-PCR analysis with twenty arbitrary lo-mer primers and agarose-gel electrophoruis,mapping of two markers flanking Lr34 Was reported. The closest marker was Pr2 (5'AGGTACCGG3,) at the 2.8 cM with logarithm of the odds to the base 10 (LOD) scores of 15.1. and the other one Pr1(5'GACCGCITGT3, was at 16.3 cM with WD scores of 6.5. Bulked segregant analysis with RAPD and linkage mapping might facilitate selection and enable gene pyramiding for leaf rust resistance in wheat breeding programs.

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