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Effect of environmental factors on vitamin B12 production by Propionibacterium freudenreichii and Bacillus megaterium [electronic resource].

By:

Abou-Taleb Khadiga, A. A

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Language: English Summary language: Arabic Description: p. 509-521Other title:

تأثير العوامل البيئية على أنتاج فيتامين ب12 بواسطة Propionibacterium freudenreichii و Bacillus megaterium [Added title page title]

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Journal of biological chemistry and environmental sciences, 2010 v. 5 (4) Part (2) [electronic resource].

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In: Journal of Biological Chemistry and Environmental Sciences 2010.v.5(4)Part(2)Summary: Effect of some environmental factors on vitaminB 12 production by Propionibacterium freudenreichii subsp. freudenreichii CCM 1857, and Propionibacterium freudenreichii subsp. shermanii P1NRC, Bacillus megaterium DSM2894, and Bacillus megaterium 1066 was investigated. Results indicated that that pH 6.6 to 6.8 were the most convenient for vitamin B12 by Bacillus megaterium, however the pH 7.0 was the best level for Propionibacterium spp. It was found that optimum incubation temperature for vitamin B12 production ranged between 30 - 35ºC. Concerning effect of aeration on biological activity and vitamin B12 productivity, results indicated that growth under static culture conditions (4 days) followed by shaking culture (4 days) for Propionibacterium spp., was the best. While growth under shaking culture conditions followed by static culture condition and again followed by shaking culture condition were suitable for Bacillus megaterium. Key words: Vitamin B12 production, Cultural conditions, Propionibacterium freudenreichii and Bacillus megaterium.

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Effect of some environmental factors on vitaminB 12 production by Propionibacterium freudenreichii subsp. freudenreichii CCM 1857, and Propionibacterium freudenreichii subsp. shermanii P1NRC, Bacillus megaterium DSM2894, and Bacillus megaterium 1066 was investigated. Results indicated that that pH 6.6 to 6.8 were the most convenient for vitamin B12 by Bacillus megaterium, however the pH 7.0 was the best level for Propionibacterium spp. It was found that optimum incubation temperature for vitamin B12 production ranged between 30 - 35ºC. Concerning effect of aeration on biological activity and vitamin B12 productivity, results indicated that growth under static culture conditions (4 days) followed by shaking culture (4 days) for Propionibacterium spp., was the best. While growth under shaking culture conditions followed by static culture condition and again followed by shaking culture condition were suitable for Bacillus megaterium. Key words: Vitamin B12 production, Cultural conditions, Propionibacterium freudenreichii and Bacillus megaterium.

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