Stability and surrogate markers of antimicrobial activity of ampicillin used in drinking water for treatment of chickens [electronic resource].

Includes references.

Physical and chemical stability of ampicillin after reconstitution in drinking water was investigated under the effect of heat at three conditions (30, 40 and 50 •C) for 8 hours. Appearance of drug solution not changed from zero time and up to 8 hours after reconstitution, while pH of the drug solution was increased from 7.21 at zero time to 7.80, 8.45 and 8.25 for three conditions respectively. Assay of ampicillin at zero time was 99.45 % and still stable for 2 hours after reconstruction (99.22, 99.11 and 99.13 %), while at 4, 6, and 8 hours, the degradation of ampicillin was recorded with final concentrations of 86.27, 86.36 and 86.19% for the three storage conditions (30, 40 and 50•C) at 8 hours respectively. After single oral administration of ampicillin before (25 mg/ kg.bwt.) and after heat stress at 50•C and storage for 8 hours (equivalent to 21.67 mg/ kg.bwt.) in clinically healthy chickens, Pharmacokinetic / pharmacodynamic integration index was 5.06 and 4.65 hours respectively.

Summary in Arabic.

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