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Survival of Azospirillum brasilense inoculated into carrier material using the immune-enzymatic elisa technique [electronic resource].

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Omar, M. N. A

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Language: English Summary language: Arabic Description: p.1-14Other title:

تقييم لقاح الازوسبييللم مع نباتات الأرز باستخدام طريقه الأليزا المناعية [Added title page title]

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Egyptian journal of agricultural research, 2008 v. 86 (1) [electronic resource].

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In: Egyptian Journal of Agricultural Research 2008.v.86 (1)Summary: Immune-enzymatic ELISA protocol was used to clarify the specificity of the polyclonal antiserum (anti-Azospirillum brasilense NO40) against Azospirillum brasilense strains WAS123 and WAS220.The optimal conditions for the serological characterization of Azospirillum brasilense strains resulted from this experiment were. bacterial count of 106 as it was the density that gave maximum optical density readings and the polyclonal antibodies dilution 10⁶ as it gave full saturation for coating the cells.

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Immune-enzymatic ELISA protocol was used to clarify the specificity of the polyclonal antiserum (anti-Azospirillum brasilense NO40) against Azospirillum brasilense strains WAS123 and WAS220.The optimal conditions for the serological characterization of Azospirillum brasilense strains resulted from this experiment were. bacterial count of 106 as it was the density that gave maximum optical density readings and the polyclonal antibodies dilution 10⁶ as it gave full saturation for coating the cells.

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