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Nucleotide sequence and secondary structure of potato spindle tuber viroid Egyptian strain [electronic resource].

By:

Mahfouze, Sherin A

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Language: English Summary language: Arabic Description: p.21-27Uniform titles:

Journal of genetic engineering and biotechnology, 2008 v. 6 (1) [electronic resource].

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In: Journal of Genetic Engineering and Biotechnology 2009.v.6(1)Summary: Potato Spindle Tuber Viroid (PSTVd - EG) was isolated from naturally infected potato plants cv. Diamond during autumn season. A reverse transcroiption-polymerase chain reaction (RT-PCR) was used for PSTVd identification using specific primer of Central Conserved Region (CCR). A major expected band with approximately 359 bp was detected in total nucleic acid extracted form PSTVd infected tissue. Southern- and dot-blot hybridizations were used to confirm the PCR-product.

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Potato Spindle Tuber Viroid (PSTVd - EG) was isolated from naturally infected potato plants cv. Diamond during autumn season. A reverse transcroiption-polymerase chain reaction (RT-PCR) was used for PSTVd identification using specific primer of Central Conserved Region (CCR). A major expected band with approximately 359 bp was detected in total nucleic acid extracted form PSTVd infected tissue. Southern- and dot-blot hybridizations were used to confirm the PCR-product.

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