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In vitro callus formation, regeneration and the active constituents content in rosmarinus officinalis l. [electronic resource].

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Bosila, Hussein A

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Language: English Summary language: Arabic Description: p.609-626Other title:

تكوين وتكشف الكالس معمليا ومحتوى المواد الفعالة فى نبات حصالبان [Added title page title]

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Journal of biological chemistry and environmental sciences, 2010 v. 5 (3) Part (2) [electronic resource].

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In: Journal of Biological Chemistry and Environmental Sciences 2010.v.5(3)Part(2)Summary: The present work was carried out to investigate in vitro callus production and active constituents content in Rosemary (Rosmarinus officicnalis L.).The results showed that modified MS (1962) basal solid medium supplemented with 4.0 and 6.0 mg/l benzyl amino purine BAP recorded the best results of callus production from node stem and shoot tip explants, callus fresh weight, dry weight and size degree. While the modified MS medium without plant growth regulators failed to format callus production and callus regeneration. The maximum level of rosmarinic acid in the callus product from node stem and shoot tip was 4.049 and 5.105 mg/g dry weight on modified MS medium supplemented with 6.0 and 8.0 mg/l BAP, respectively. The shootlets product from node stem and shoot tip in medium supplemented with 6.0 and 8.0 mg/l BAP+5.0 mg/l GA₃. Were formed the best levels, 24.293 and 30.630 mg/g dry weight, respectively. The highest percentage of regeneration was for node stem and shoot tip cultures on modified MS medium supplemented with 6.0 mg/l BAP+5.0 mg/l GA₃ which gave 54.55 and 65.0 %, number of shootlets (5.0 and 13.6), length of shootlets (1.8 and 3.0 cm) respectively in vitro culture. Increase of BAP concentration from 1.0 to 4.0 mg/l combined with 5.0 mg/l GA3 significant on essential oil % in shootlets produced from node stem as level α-pinene, camphene, limonene, 1,8-cineol and linalool respectively.

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The present work was carried out to investigate in vitro callus production and active constituents content in Rosemary (Rosmarinus officicnalis L.).The results showed that modified MS (1962) basal solid medium supplemented with 4.0 and 6.0 mg/l benzyl amino purine BAP recorded the best results of callus production from node stem and shoot tip explants, callus fresh weight, dry weight and size degree. While the modified MS medium without plant growth regulators failed to format callus production and callus regeneration. The maximum level of rosmarinic acid in the callus product from node stem and shoot tip was 4.049 and 5.105 mg/g dry weight on modified MS medium supplemented with 6.0 and 8.0 mg/l BAP, respectively. The shootlets product from node stem and shoot tip in medium supplemented with 6.0 and 8.0 mg/l BAP+5.0 mg/l GA₃. Were formed the best levels, 24.293 and 30.630 mg/g dry weight, respectively. The highest percentage of regeneration was for node stem and shoot tip cultures on modified MS medium supplemented with 6.0 mg/l BAP+5.0 mg/l GA₃ which gave 54.55 and 65.0 %, number of shootlets (5.0 and 13.6), length of shootlets (1.8 and 3.0 cm) respectively in vitro culture. Increase of BAP concentration from 1.0 to 4.0 mg/l combined with 5.0 mg/l GA3 significant on essential oil % in shootlets produced from node stem as level α-pinene, camphene, limonene, 1,8-cineol and linalool respectively.

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