In vitro fertilization and development of in vitro versus in vivo matured rabbit Oocytes [electronic resource].

Includes references.

This study was carried out to evaluate the effect of adding two types of serum, bovine serum albumin (BSA) and doe rabbit serum (DRS) to maturation tissue culture medium (TCM-199) on the in vitro maturation rate of follicular oocytes of rabbits, and to, evaluate the success rate of in vitro fertilization and development of in vivo versus in vitro mature rabbit oocytes. About 245 immature complete cumulus oocytes were recovered immediately from ovaries of sacrijied rabbit does treated with PMSG (75 IU) for 48 h. Maturation medium (TCM-199) supplemented with 10% DRS, 6% BSA or without serum (control) were used. In vitro (n= 245) and in vivo (n=264, collected from oviduct of does sacrijied after 12 h of 0. 02 ml GnRH injection) mature oocytes were used for in vitro fertilization and embryo development evaluation. Results revealed that TCM-199 plus DRS increased the percentage of oocytes at M I (25 vs.15%, P<0.05) and M II (55 vs. 47.5%, P>0.05) as compared to control TCM, respectively. However, TCM-199 plus BSA had more positive effect on in vitro maturation of oocyte in term of the lowest percentage of oocytes at germinal vesicles and germinal vesicles break down (0.0 and 9.4%) and the highest one (73%, P<0.05) at M II. Fertilization rate was higher (P<O. 05) for in vivo than in vitro mature oocytes (86. 3 vs. 62. 5%). Percentage of embryos 4-cell stage decreased (18.3 vs. 34. 7%, P<O. 05) and at morula stage increased (30.5 vs. 16%, P<0.05) for in vivo as compared to in vitro matured oocytes. However, the percentages of embryos at 2-cell and 8 16-cell stages between in vivo and in vitro matured oocytes were not different.

Summary in Arabic.

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