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In vitro characterization and pathogenesis of Egypt /Beni-Suef/01; a novel genotype of infectious bronchitis virus [electronic resource].

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Abdel-Moneim, A. S

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Language: English Summary language: Arabic Description: p.127-133Uniform titles:

Beni-Suef veterinary medical journal, 2005 v. 15 (2), Special issue [electronic resource]:

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In: Beni-Suef Veterinary Medical Journal. Beni-Suef Vetrinary Medicen Journal November 2005, 4th Scientific conference 2005.v.15(2)(Special issue)Summary: In vitro characterizations of infectious bronchitis virus (Egypt/Beni-Suef/01 strain) were studied. Heat stability at 56 'C for 5, 10, 15, 20, and 30 min, UV irradiation for 10, 15,20, and 30 min, chloroform sensitivity, pH stability, ana E/M examination were screened. Two-week-old commercial chickens were inoculated intraocularly with Egypt/Beni-Suef/01. At days I, 3, 5, 7, II and 14 days post¬ infection, three birds were euthanatized, and virus antigens were screened using immunofluorescence. Histopathology of both trachea & kidney was also performed. Egypt /Beni-Suer/Ol was found to be sensitive to 56'C for 5 min, UV for 10 min and chloroform but resistant to pH 3 and pH 9. It showed typical morphology of coronaviruses by E/M. The virus was found to induce mild respiratory signs and sever renal changes. Immunoflourescence staining showed viral antigen in larynx, trachea, thymus, lung, liver, spleen, renal tubules and glomeruli as well as bursa of Fabricius but not in pancreas. Marked histopathological lesions were also detected in both trachea and kidneys.

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In vitro characterizations of infectious bronchitis virus (Egypt/Beni-Suef/01 strain) were studied. Heat stability at 56 'C for 5, 10, 15, 20, and 30 min, UV irradiation for 10, 15,20, and 30 min, chloroform sensitivity, pH stability, ana E/M examination were screened. Two-week-old commercial chickens were inoculated intraocularly with Egypt/Beni-Suef/01. At days I, 3, 5, 7, II and 14 days post¬ infection, three birds were euthanatized, and virus antigens were screened using immunofluorescence. Histopathology of both trachea & kidney was also performed. Egypt /Beni-Suer/Ol was found to be sensitive to 56'C for 5 min, UV for 10 min and chloroform but resistant to pH 3 and pH 9. It showed typical morphology of coronaviruses by E/M. The virus was found to induce mild respiratory signs and sever renal changes. Immunoflourescence staining showed viral antigen in larynx, trachea, thymus, lung, liver, spleen, renal tubules and glomeruli as well as bursa of Fabricius but not in pancreas. Marked histopathological lesions were also detected in both trachea and kidneys.

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