GC-MS and HPLC analyses of phytochemical compounds from Withania Somnifera L. leaves extract [electronic resource]
Language: English Summary language: Arabic Description: p. 11-17Other title:- التحليل الكروماتوجافى الغازي المتصل بالمطياف الكتلى والتحليل الكروماتوجرافى السائل عالي الكفائة لتحليل المركبات الكيميائية من مستخلص أوراق نبات ال Withania Somnifera L [Added title page title]
- Alexandria journal of agricultural sciences, 2022 v. 67 (1) [electronic resource]
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Includes bibliographic reference
In the present work, the shrub W. somnifera was used as a source for the extraction of
bioactive molecules. Leaves were extracted by ethanol and analyzed by Gas Chromatography
-Mass Spectrometry (GC-MS) and High-Performance Liquid Chromatography (HPLC). By GC-MS
analysis, the highest identified compounds were 2,6-dimethyl-N-(2-methyl-?-phenylbenzyl)
aniline, 4-Aminoheptane, n-undecanophenone, 2-cyclohexyl-4-hydroxymethyl-octahydrobenzo
[e][1,2] oxazine-3-carbonitrile, and octadecanoic acid 3-octadecyloxypropyl ester,
with percentages of 19.43%, 7.51%, 4.47%, 4.34%, and 3.89%, respectively. HPLC analysis
identified quercetin and 7-hydroxyflavone with concentrations of 12.36 and 15.14 mg/mL,
respectively, as two flavonoid compounds. This work showed that W. somnifera leaf extract
owns several bioactive compounds that could be useful and have different applications,
such as antimicrobial activities.
Key words: Withania somnifera leaves; HPLC; GC-MS; quercetin; 7-hydroxyflavone.
Summary in Arabic
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