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Genetic improvement of dextranase production by penicillum funiculosum via mutation induction and protoplast fusion [electronic resource]..

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Talkhan, F.N

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Language: English Summary language: Arabic Description: p. 791-804Other title:

التحسين الوراثي لإنتاج الديكسترينيز فطر البنسليوم فونيكيولوزم من خلال استحداث الطفرات والدمج البروتوبلاستي [Added title page title]

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Annals of agricultural science, 2002 v. 47 (2) [electronic resource]..

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In: Annals of Agricultural Science 2002.v.47(2)Summary: The fungal strain Penicillium funiculosum NRPL- 6014 was exposed to UV- irradiation at 20cm distance from the lamp for .4, 6, 8, 10 and 15 minutes. About 100 mutants were isolated, as auxotropbic or/and morphological variants, and screened for dextranase activity qualitatively. Forty mutants proved to be enzyme producers and were chosen for quantitative determination of the enzyme. Results indicated that 30 mutants produced the enzyme more than the wild type, 6 of which gave dextranase activity two fold or more of the original parent, whereas the remaining 10 mutants produced the enzyme less than the W.T strain. Four different mutants were chosen for protoplast fusion, according to enzyme activity, antifungal agents response and morphological variants, in three cross combinations (a), (b) and (c) in which mutant No. 18 was common parent.

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The fungal strain Penicillium funiculosum NRPL- 6014 was exposed to UV- irradiation at 20cm distance from the lamp for .4, 6, 8, 10 and 15 minutes. About 100 mutants were isolated, as auxotropbic or/and morphological variants, and screened for dextranase activity qualitatively. Forty mutants proved to be enzyme producers and were chosen for quantitative determination of the enzyme. Results indicated that 30 mutants produced the enzyme more than the wild type, 6 of which gave dextranase activity two fold or more of the original parent, whereas the remaining 10 mutants produced the enzyme less than the W.T strain. Four different mutants were chosen for protoplast fusion, according to enzyme activity, antifungal agents response and morphological variants, in three cross combinations (a), (b) and (c) in which mutant No. 18 was common parent.

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