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Detection of reticuloendothellosis virus as contaminant of fowl pox vaccines [electronic resource].

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Abd El Hamid, H. S

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Language: English Summary language: Arabic Description: p.497-524Other title:

دراسة عن الكشف عن فيروس الرتيكلواندوثيليوزيس كملوث فيروسي في لقاح جدري الطيور [Added title page title]

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Veterinary medical journal, 2009 v.57 (3), Part 2 [electronic resource]:

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In: Veterinary Medical Journal 2009.v.57(3)Summary: Results revealed that: only one imported vaccine sample gave positive results by PCR test a roduct of291- bp was obtained by the vaccine sample. Serum samples collected from positive vaccine batch were tested for REV antibodies by ELISA test and the sera were positive. Histopathological examination for liver, spleen and bursa of fabricius revealed the presence of tumor cells in the examined organs and these changes confirmed the results obtained by PCR and ELISA tests and indicated that the sample is contaminated with REV. The data clearly indicated that screening of all commercial poultry vaccines to be virus free is an important factor in assuring the biosafety of animal vaccines.

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Results revealed that: only one imported vaccine sample gave positive results by PCR test a roduct of291- bp was obtained by the vaccine sample. Serum samples collected from positive vaccine batch were tested for REV antibodies by ELISA test and the sera were positive. Histopathological examination for liver, spleen and bursa of fabricius revealed the presence of tumor cells in the examined organs and these changes confirmed the results obtained by PCR and ELISA tests and indicated that the sample is contaminated with REV. The data clearly indicated that screening of all commercial poultry vaccines to be virus free is an important factor in assuring the biosafety of animal vaccines.

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