Physical finger print and biochemical studies on the insecticide "lufenuron" against the nile tilapia, Oreochromis niloticus and its residue levels in tissues [electronic resource].

Includes reference.

Physical finger prints of Lufenuron (active ingredient and its formulation Match 5% EC) were studied. Lufenuron was a colorless crystals with melting point 170°C and 99% as purity. Also Match 50% EC comply the FAOIWHO specification of EC where it was slightly acidic with pH 4.88, flash point over 75°C, refractive index 1.489, density about 0.932 gm/cm3 and it passed cold and hot cold test for 1 hr at 0°C and hot storage at 54°C for 3 days without any degradation or sedimentation. The physicochemical properties of spray solutions (soft, hard and tap water) showed that a good emulsion stability and decrease the surface tension of water from 72 dynelcm to 37 dyne\cm which gave high degree ofwettability on the treated surface. The adverse effects due to exposure of the Nile tilapia, Oreochromis niloticus to sublethal concentration (1/10 LC₅₀) of Lufenuron insecticide (i.e. technical and formulation form) for 21 days, then allowed to a recovery for seven days, were investigated through some biochemical parameters and tissues residues determinations. The results showed a significant increase of serum glucose levels, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities, in addition to creatinine levels, while the total serum protein and total serum lipid showed a significant decrease. The residual analysis exhibited that the concentration of Lufenuron residues in the liver were 0.506 and 1.505 ppm and in fish muscles were 0.281 and 0.417 ppm after 21 days oftreatments as active ingredient and formulation form, respectively. However higher concentration of residues were detected in the fish gills (3.139 and 1.726 ppm) after one day of treatment. Furthermore, the higher persistent rate of the tested insecticide "Lufenuron" in the water was detected after 24 hours of application and concentration losses oftested insecticide were increased by time.

Summary in Arabic.

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