Effect of dietary glutathione, aluminosilicate and tafla on laying hens during aflatoxicosis [electronic resource].

Includes references.

An experiment was conducted to evaluate effect of dietary tripeptide glutamate (reduced glutathione (GSH)) as antioxidant, tfjla (TF), and hydrated sodium calcium aluminosilicate (HSCAS) as sorbent materials to reduce aflatoxicosis in chickens. A total number of 371 (350 laying hens+21 cocks) thirty-wk old EI-Salam chickens were randomly divided into 7 groups; each group included 5 replicates of 10 hens each and housed in metallic batteries. The remaining 21 cocks were also divided into 7 groups of 3 cocks each and housed separately for semen collection. Birds were fed practical corn-soybean meal basal diet with or without 1 ppm aflatoxin BJ (AFBJ alone or plus either 5ppm GSH. 0.6% TF, 0.5% HSCAS. 0.6% TF+ 5ppm GSH or 0.5% HSCAS+ 5ppm GSH to form 7 diets fed from 30 to 38 wks old. Results show that contamination of basal diet with 1 ppm AFB1 for 8 wks decreased (P<.01) feed intake (25.1%), egg production (42.8%), egg weight (22.3%), shell thickness (32.6%), fertility (21.9%), hatchability. of fertile eggs (20%), economic efficiency (EE, 38. 5%), liver vitamin A (29.1%), blood hemoglobin (35.6%), serum albumin (68%) and total lipids (51%), increased relative liver weight (138.8%), liver lipids (141.9%), blood total leucocytes (WEC's) (28%) and lymphocytes (27.2%) counts, serum enzymatic activities of AST (64%) and ALT (69%), and deposited AFBI residues in livers (68 ng/g), egg yolk- (52 ng/g) and muscles (36 ng/g) compared to the controls. Adding TF or HSCAS separately into AF diet recorded similar protection effects averaged 45-56% against ajlatoxicosis for the studied traits. Including GSH alone into the AF diet resulted in a little protective effect against AF diet for the all studied traits, except AST and ALT activities that showed a significant protective effect (20-28%). However, GSH together with TF or HSCAS significantly negated the adverse effects of AF diet for all studied traits. Supplementing sorbent materials plus GSHwith AF-diet improved EE 80.97%for TF+GSH and 74.87%for HSCAS+GSH compared to AF-diet. There were mortalities only among two groups fed basal diet with AFBJ alone (10%) and AFBJ+GSH (6%). The present study revealed that TF presented similar protective effect for studied traits and EE as HSCAS. Adding GSH as antioxidant together with TF or HSCAS, to AFBJ contaminated diet significantly negated aflatoxicosis in the laying hens.

Summary in Arabic.

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