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Tissue culture organogensises in soybean [electronic resource].

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Nasr, M. I

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Language: English Summary language: Arabic Description: P. 5201-5208Other title:

تكوين الأعضاء النباتية بواسطة زراعة الأنسجة فى فول الصويا.‪ [Added title page title]

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Mansoura University journal of agricultural sciences, 2005 v. 30 (9) [electronic resource].

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In: Mansoura Unviersity Journal of Agricultural Sciences 2005.v.30(9)Summary: The aim of this study was to initiate and maintain callus organogenesis cultures of soybean and evaluate the response of seven soybean genotypes in callus formation. The genotypes were selected on the basis of their reaction to cotton leaf worm infection. This investigation was conducted in the field at Giza research station in 2003 and 2004seasons. Immature embryos (with 0.5-10 mm long) were excised from immature seeds and used as ex-plant. The basal Murashige and Skoog (MS) medium with addition of 4 times of micronutrients, Proline (1381 mgll), NAA (0.0372 mg/l) and SAP (2.996 mg/I) gave complete satisfaction for callus production. Formation of shoots and roots were succeeded and rooted plantlets were transplanted to pots in green house. The results indicated that the genotype L86K-73 was the best in response to tissue culture technique in soybean. Callus growth rate was positively and significantly correlated with each of number of shoots/callus and number of roots. Therefore, both characters could be used to predict succeeding of callus growth in soybean.

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The aim of this study was to initiate and maintain callus organogenesis cultures of soybean and evaluate the response of seven soybean genotypes in callus formation. The genotypes were selected on the basis of their reaction to cotton leaf worm infection. This investigation was conducted in the field at Giza research station in 2003 and 2004seasons. Immature embryos (with 0.5-10 mm long) were excised from immature seeds and used as ex-plant. The basal Murashige and Skoog (MS) medium with addition of 4 times of micronutrients, Proline (1381 mgll), NAA (0.0372 mg/l) and SAP (2.996 mg/I) gave complete satisfaction for callus production. Formation of shoots and roots were succeeded and rooted plantlets were transplanted to pots in green house. The results indicated that the genotype L86K-73 was the best in response to tissue culture technique in soybean. Callus growth rate was positively and significantly correlated with each of number of shoots/callus and number of roots. Therefore, both characters could be used to predict succeeding of callus growth in soybean.

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