Detection, purification, and identification of siderophores produced by Pseudomonas fluorescens isolates using SDS-PAGE and HPLC [electronic resource].

Includes references.

Three isolates of Pseudomonas fluorescens were isolated from the soil rhizosphere of potato growing fields and identification was confirmed by comparison of the morphological traits and physiological characteristics according to Murray et al., 1984 and Klement et al.,(1990). P. fluorescens isolates were tested to prove their ability in production of siderophores. Chrome Azurol Sulfonate assay (CAS assay) test was used to detect the presence of siderophores. Formation of an orange halo around a well bored into the CAS plate, was taken as an indication for the presence of siderophores. Concerning the optimization of conditions for highest production of siderophores on different media, the results revealed that king's 8 medium, exhibited the highest production of sidrophores. Also, data showed that pH 7 and 27 °C were the best in this respect while at pH 6 and 8 sidrophores production completely inhibited.

Summary in Arabic.

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