Isolation, identification and molecular characterization of halo and thermo tolerant bacteria from costal ridge of Mediterranean in Egypt [electronic resource].

Includes references.

Costal ridge of Mediterranean and the regions near by it, like Mallahat Maryut, Bourg El-Arab and El-Amiriya in the north of Egypt are suitable places for the isolation of halo- and thermo-tolerant microorganisms. This study presents isolation, identification and molecular characterization of halotolerant and thermophilic bacteria. Ten bacterial isolates were isolated using enrichment techniques, up to 30% (W/V) NaCl and 75°C incubation temperatures. Isolates were identified according to Bergey's Manual as Bacillus species, six isolates were identified as Bacillus thuringiensis and the other four isolates were B. polymyxa. Protein profiling of the Bt isolates and the PCR detection of cry genes showed the presence of lepidopteran cry1C gene. On the protein synthesis level, the protein profiling of cells grown in 5.2 M NaCl showed or/ disappeared a set of high-salt related proteins. On the other hand, the other 4 isolates of B. polymyxa were able to grow at 75°C. Protein profiling and the PCR detection of glnB & nifD genes revealed the presence of these genes. Thus B. polymyxa isolates could be used to fix nitrogen on the crops cultivated in hot regions.

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