Genetic diversity study in the genus Latllyrus using RAPD analysis [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: p.11-20Uniform titles:
  • Journal of genetic engineering and biotechnology, 2008 v. 6 (1) [electronic resource].
Subject(s): Online resources: In: Journal of Genetic Engineering and Biotechnology 2009.v.6(1)Summary: Molecular markers based on PCR analysis using arbitrary 10-mer primers (RAPD) was used to identify and assess the genetic diversity of accessions of seven Lathyrus species: L. aphaca, L. clymenum, L. ochrus, L. annuus, L. cicera and L. sativa tingitanus. A total of 253 amplified products ranging in size from 300 bp to 3100 bp were generated by PCR amplification using 18 primers. Genetics similarities were estimated on the basis of pair-wise comparisons of RAPD marker analysis and dendrogram of genetic relationship between species were constructed. The RAPD data obtained were sufficient to distinguish between accessions and to separate these accessions by clustering them according to species. A high degree of genetic variability was recorded between studied species.
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Molecular markers based on PCR analysis using arbitrary 10-mer primers (RAPD) was used to identify and assess the genetic diversity of accessions of seven Lathyrus species: L. aphaca, L. clymenum, L. ochrus, L. annuus, L. cicera and L. sativa tingitanus. A total of 253 amplified products ranging in size from 300 bp to 3100 bp were generated by PCR amplification using 18 primers. Genetics similarities were estimated on the basis of pair-wise comparisons of RAPD marker analysis and dendrogram of genetic relationship between species were constructed. The RAPD data obtained were sufficient to distinguish between accessions and to separate these accessions by clustering them according to species. A high degree of genetic variability was recorded between studied species.

Summary in Arabic.

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