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A novel SNP in exon 5 of the GHR gene and its association with average daily gain in Beheiry Egyptian water buffaloes (Bubalus Bubalis) [electronic resource].

By:

El-Shahata, Mohammad

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Language: English Summary language: Arabic Description: p.261-271Other title:

تحديد طفرة جديدة في الاكسون من جين مستقبلات هرمون النمو وارتباطه مع متوسط الزيادة اليومية في بحيري جاموس المياه المصرية [Added title page title]

Uniform titles:

kafrelsheikh veterinary medical journal, 2014 v. 12 (1) [electronic resource].

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In: Kafrelsheikh Veterinary Medical Journal 2014.v.12(1)Summary: The polymorphisms in growth hormone receptor gene (GHR) were determined by advanced molecular techniques as well as their association with growth traits in Beheiry Egyptian water buffalo. Therefore, the objective of this study was to detect GHR gene polymorphisms in Beheiry Egyptian water buffalo. Our results revealed the presence of SNP C7 42T in Beheiry Egyptian water buffalo by using PCR and SSCP methods followed by nucleotide sequencing to identify its different allelic patterns in the same animals under study. Three genotypes (CC, TC and TT) were identified by SSCP technique. Statistical analysis showed that, genotype TT was significantly higher than CT and CCgenotypes at age from 6 to 12 month (P< 0.05).

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The polymorphisms in growth hormone receptor gene (GHR) were determined by advanced molecular techniques as well as their association with growth traits in Beheiry Egyptian water buffalo. Therefore, the objective of this study was to detect GHR gene polymorphisms in Beheiry Egyptian water buffalo. Our results revealed the presence of SNP C7 42T in Beheiry Egyptian water buffalo by using PCR and SSCP methods followed by nucleotide sequencing to identify its different allelic patterns in the same animals under study. Three genotypes (CC, TC and TT) were identified by SSCP technique. Statistical analysis showed that, genotype TT was significantly higher than CT and CCgenotypes at age from 6 to 12 month (P< 0.05).

Summary in Arabic.

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