Genomic identification of Pasteurella multocida isolated from turkey flock in Egypt early 2008 [electronic resource]

Includes references.

Molecular detection and differentiation of Paslellrea multocida strain involved in a separate fowl cbolera outbreak in a turkey flock farm. located in El-Menon. Governorate, Egypt early 2008 was investigated. The isolated strain was compared with an Egyptian Pasteurella multocida isolate that was previously isolated from turkey flock during last decade besides fOUf vaccinal strain (A:S, A:8, A:9 and D:2) DB phenotypic and genotypic characterization basis. Phenotypically all the strains were similar as all the strains productionn haemolytic colonies on blood agar, and all the strains revealed similar biochemial behaviour. On the other hand, the genomic typing of all the stains using rep-PCR techniques (repetitive BOX elements, enterobacterial repetitive intergenic consensus (ERIC) and repetitive eltragenic palindromic (REP) polym.erase chain reaction (peR») differentiated the six PasteureUa mulJocida strains mto six different profiles. The molecular identity between the Pasteurella multocida 2008 strain and the previously isolated strain was 76.6 % and were ranged frOID 65.2°;' to 79.2°;' with the 4 vaccinal strains. These results reported the continuous mutations of the field Pasteurella multocida strains among poultry flocks in Egypt ibdicating the urgent need for the frequnt and continuous molecular epidemiological investigations of fowl cholera outbreaks in various poultry nocks to detect these new strains and updatetbe fowl cholera vaccines.

Summary in Arabic.

1