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Characterization of S.aureus isolated from subclinical bovine mastitis [electronic resource].

By:

Enany, M.E

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Language: English Summary language: Arabic Description: p.144-150Other title:

توصيف الميكروب العنقودى الذهبى المعزول من التهاب الضرع السريرى فى الماشية [Added title page title]

Uniform titles:

Zagazig veterinary journal, 2011 v. 39 (1) [electronic resource].

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In: Zagazig Veterinary Journal 2011.v.39(1)Summary: A total of 480 quarter milk samples were collected from 120 apparently healthy dairy animals (50 local breed buffaloes and 70 Friesian cows) from different farms at Ismailia Governorate. Samples were subjected to California Mastitis Test where the total percentage of subclinical mastitis was 48.75%. Positive samples for CMT were subjected to bacteriological examinations where the percentage of S.aureus was 35.9%. Antibiotic sensitivity test was carried out against several drugs where 64.28% of Saureus isolates were resistant to penicillin. PCR protocol was applied for amplification and detection of both 16S rRNA and coagulase genes in 28 S. aureus isolates to confirm their diagnosis and their virulence.

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A total of 480 quarter milk samples were collected from 120 apparently healthy dairy animals (50 local breed buffaloes and 70 Friesian cows) from different farms at Ismailia Governorate. Samples were subjected to California Mastitis Test where the total percentage of subclinical mastitis was 48.75%. Positive samples for CMT were subjected to bacteriological examinations where the percentage of S.aureus was 35.9%. Antibiotic sensitivity test was carried out against several drugs where 64.28% of Saureus isolates were resistant to penicillin. PCR protocol was applied for amplification and detection of both 16S rRNA and coagulase genes in 28 S. aureus isolates to confirm their diagnosis and their virulence.

Summary in Arabic.

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