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Genetic differentiation of Erwinia carotovora subsp. carotovora and subsp. atroseptica [electronic resource].

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Khalil, Hadeel M. M

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Language: English Summary language: Arabic Description: P.29-44Other title:

الاختلافات الوراثية فى البكتريا إيروينيا كاروتوفورا تحت نوع كارتوفورا وإيروينيا كاروتوفورا تحت انواع أتروسبتيكا [Added title page title]

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Assiut journal of agricultural sciences, 2014 v. 45 (4) [electronic resource].

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In: Assiut Journal of Agricultural Sciences 2014.v.45(4)Summary: Erwinia carotovora subsp. atroseptica (Eca), the causes of blackleg of potato, is closely resembles to E. carotovora subsp. carotovora (Ecc), the causes of bacterial soft rot of potato, in many physiological and biochemical characteristics. Nine isolates were obtained from potato tubers collected from different localities of El-Minia, Assiut and Sohag Governorates and subjected to this investigation. Three different molecular techniques were employed to differentiate the two subsp. of Erwinia carotovora. The primers used in RAPD-PCR technique generated an unique distinct bands which could be used as genetic markers to distinguish the isolates in respect of their sub species (carotovora or atroseptica) or their virulence (high or low virulence). Also, the 16s rRNA gene sequences was obtained from the bacterial isolates of Ecc and Eca. Keywords: RAPD; SDS-PAGE; 16s rRNA gene; Erwinia carotovora.

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Erwinia carotovora subsp. atroseptica (Eca), the causes of blackleg of potato, is closely resembles to E. carotovora subsp. carotovora (Ecc), the causes of bacterial soft rot of potato, in many physiological and biochemical characteristics. Nine isolates were obtained from potato tubers collected from different localities of El-Minia, Assiut and Sohag Governorates and subjected to this investigation. Three different molecular techniques were employed to differentiate the two subsp. of Erwinia carotovora. The primers used in RAPD-PCR technique generated an unique distinct bands which could be used as genetic markers to distinguish the isolates in respect of their sub species (carotovora or atroseptica) or their virulence (high or low virulence). Also, the 16s rRNA gene sequences was obtained from the bacterial isolates of Ecc and Eca. Keywords: RAPD; SDS-PAGE; 16s rRNA gene; Erwinia carotovora.

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