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New approach to in-vitro propagation of babesia bovis in low serum content [electronic resource].

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Romany, M M

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Language: English Summary language: Arabic Description: p.97-104Other title:

محاولات جديدة لإكثار طفيل البابيزيا بوفيز معملياُ فى أقل كمية سيرم ممكنة [Added title page title]

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Zagazig veterinary journal, 2010 v. 38 (2) [electronic resource].

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In: Zagazig Veterinary Journal 2010.v.38(2)Summary: In-vitro propagation of B. bovis using microaerophilous stationary phase (MASP) were investigated. Medium I 99 (Med199) containing 40% bovine serum showed good propagation of the parasites represented by the percent of infected red blood corpuscles. Reduction in bovine serum content in Medl99 to 20% or less resulted in lower growth of the parasite. LH-l medium containing 20% bovine serum was gradually replaced Medl99 containing 40% serum. After ten serial passages, the parasite was adapted for growth in the LH-l medium with 20% serum. Other successful trials to reduce serum content in the culture to 10% were done.

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In-vitro propagation of B. bovis using microaerophilous stationary phase (MASP) were investigated. Medium I 99 (Med199) containing 40% bovine serum showed good propagation of the parasites represented by the percent of infected red blood corpuscles. Reduction in bovine serum content in Medl99 to 20% or less resulted in lower growth of the parasite. LH-l medium containing 20% bovine serum was gradually replaced Medl99 containing 40% serum. After ten serial passages, the parasite was adapted for growth in the LH-l medium with 20% serum. Other successful trials to reduce serum content in the culture to 10% were done.

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