Determination of some molecular markers associated with sugar content in sweet sorghum [electronic resource].

Includes reference.

The present study was carried out to evaluate three inbred lines of sweet sorghum for their performances to sugar content at the field. These three inbred were chosen as a high sugar content (Wiley) and low sugar content (Roma and Rex) for hybridization to obtain the F1 plants and then selfed to obtain the F2 plants for each cross of Roma X Wiley and Wiley X Rex. These three parental lines and their segregated F2 plans for each cross were evaluated for their performances to sugar content by recording two sugar content – related traits such as sucrose and total soluble solids percentages as well as days to a 50% of heading traits. Bulks of the two extreme F2 plant groups (the highest and the lowest sugar content groups) for each cross and the three parental line plants were tested against seven RAPD, five ISSR and three SSR primers to determine some molecular markers for sugar content in sweet sorghum. However, the three SSR primers were monomorphic, while the others were polymorphic which only five RAPD primers out of them developed molecular markers for sugar content. Five positive markers in the two crosses as well as two positive markers in the cross of Wiley X Rex were detected. On the other hand, three negative markers in the two crosses as well as one negative marker in the cross of Wiley X Rex were also detected.

Summary in Arabic.

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