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In vitro propagation of chrysanthemum (Dendranthema grandiflora Tzvelev) cv. "Euro White" using nodal segment explants [electronic resource].

By:

Gaber, M. K

Contributor(s):

Language: English Summary language: Arabic Description: p.644-659Other title:

الاكثار المعملي الدقيق لنبات الاراولا لصنف يورو وايت باستخدام القطع البرعمية الساقية [Added title page title]

Uniform titles:

Journal of the advances in agricultural researches, 2012 v. 17 (3) [electronic resource].

Subject(s):

Chrysanthemum -- Propagation

Online resources:

Full Text Article.

In: Journal of the Advances in Agricultural Researches 2012.v.17(3)Summary: An efficient plant tissue culture protocol for chrysanthemum (Dendranthema grandiflora Tzvelev) plant "Euro White" cv. has been developed trom the nodal cuttings. Nodal cuttings, after being disinfected superficially, sterilized with 1.0% mercuric chloride for five minutes and then were rinsed thrice with autoclaved distilled water. For initiation stage, surfaoe sterilized explants were cultured on Murashige and Skoog (MS) medium augmented with varied concentrations of both applied plant growth regulants kinetin (KIN) at 0.0 (nil), 0.5, 1.0 and 2.0 mg/l, and in combination with naphthalene acetic acid (NAA) at 0.0 (nil), 0.5 and 1.0 mg/l. Different parameters including mean number of shoots, shoot length (cm), mean number of leaflets, mean number of nodes and mean number of roots formed per propagule were studied during the course of this stage.

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An efficient plant tissue culture protocol for chrysanthemum (Dendranthema grandiflora Tzvelev) plant "Euro White" cv. has been developed trom the nodal cuttings. Nodal cuttings, after being disinfected superficially, sterilized with 1.0% mercuric chloride for five minutes and then were rinsed thrice with autoclaved distilled water. For initiation stage, surfaoe sterilized explants were cultured on Murashige and Skoog (MS) medium augmented with varied concentrations of both applied plant growth regulants kinetin (KIN) at 0.0 (nil), 0.5, 1.0 and 2.0 mg/l, and in combination with naphthalene acetic acid (NAA) at 0.0 (nil), 0.5 and 1.0 mg/l. Different parameters including mean number of shoots, shoot length (cm), mean number of leaflets, mean number of nodes and mean number of roots formed per propagule were studied during the course of this stage.

Summary in Arabic.

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