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Studies on transformation of resistant plasmid between Salmonella and E. coli [electronic resource].

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Ibrahim, Hanan M

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Language: English Summary language: Arabic Description: p.777-786Other title:

دراسات على إنتقال البلازميد المقاوم بين السالمونيلا والإيشريشيا كولاى [Added title page title]

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Veterinary medical journal, 2006 v.54 (4) [electronic resource].

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In: Veterinary Medical Journal 2006.v.54(4)Summary: Plasmid profile analysis of 10 multiple drug resistant Salmonella serovars with (resistant to ampicillin, chloramphenicol, oxytetracycline, nalidixic acid and streptomycin) was performed using agarose gel electrophoresis. There were 8 isolates harbouring plasmid with molecular weight ranged from 16 - 31.5 kbp while the plasmid of 2 isolates could not be detected. Successful transformation of resistant plasmid was done between S. Kentucky and S. Tvphimurium as donor bacteria and E. coli XLI as recipient bacteria by electroporation. Transconjugants were obtained by conju!;ation assay.between multiple drug resistant (MDR) S. Typhimurium as donor bacteria and each of S. Enteritidis, S. Typhi. E. coli: XLI and E. coli: O157H7 as recipient bacteria Salmonella enterica is a major endemic and epidemic pathogen in animals and human worldwide and has a great effect on animal production.

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Plasmid profile analysis of 10 multiple drug resistant Salmonella serovars with (resistant to ampicillin, chloramphenicol, oxytetracycline, nalidixic acid and streptomycin) was performed using agarose gel electrophoresis. There were 8 isolates harbouring plasmid with molecular weight ranged from 16 - 31.5 kbp while the plasmid of 2 isolates could not be detected. Successful transformation of resistant plasmid was done between S. Kentucky and S. Tvphimurium as donor bacteria and E. coli XLI as recipient bacteria by electroporation. Transconjugants were obtained by conju!;ation assay.between multiple drug resistant (MDR) S. Typhimurium as donor bacteria and each of S. Enteritidis, S. Typhi. E. coli: XLI and E. coli: O157H7 as recipient bacteria Salmonella enterica is a major endemic and epidemic pathogen in animals and human worldwide and has a great effect on animal production.

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