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Differentiation among Ralstonia solanacearum isolates causing potato bacterial wilt by random amplified polymorphic DNA [electronic resource].

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Hassan, Maggie E. M

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Language: English Summary language: Arabic Description: p.1505-1520Other title:

التفرقة بين عزلات رلستونيا سولانسيرم المعزولة من البطاطس والمسببة للذبول البكتيرى بواسطة التضاعف العشوائى للحامض النووى [Added title page title]

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Egyptian journal of agricultural research, 2003 v. 81 (4) [electronic resource].

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In: Egyptian Journal of Agricultural Research 2003.v.81(4)Summary: Ten Ralstonia solanacearum isolates were obtained from Spunta and Nicola potato cultivars . PCR-based RAPD analysis was carried out, using four primers, on agarose and polyacrylamide gels as support media. Only, the primer 6-d (AACGCGCAAC)-3 differentiated the isolates recovered from the two cultivars, others did not. In general, separation on poly-acrylamide and staining with silver nitrate gave better resolution than those on agarose and stained with ethidium bromide. Further investigations are needed using greater number of isolates, from different potato cultivars and /or different sources, to confirm the result as well as the possible effect of the host cultivars on the properties of bacterial DNA.

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Ten Ralstonia solanacearum isolates were obtained from Spunta and Nicola potato cultivars . PCR-based RAPD analysis was carried out, using four primers, on agarose and polyacrylamide gels as support media. Only, the primer 6-d (AACGCGCAAC)-3 differentiated the isolates recovered from the two cultivars, others did not. In general, separation on poly-acrylamide and staining with silver nitrate gave better resolution than those on agarose and stained with ethidium bromide. Further investigations are needed using greater number of isolates, from different potato cultivars and /or different sources, to confirm the result as well as the possible effect of the host cultivars on the properties of bacterial DNA.

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