Isolation and characterization of variant strains of mycoplasma galusepticum (MG) from broiler breeder chicken and rats [electronic resource].

Includes reference.

The serological profile of Mycoplasma gallisepticum (MG) in a broiler breeder flock showed negative result until 30 weeks of age, where it . became MG positive ( 100%), when tested at both of 40 and 55 weeks of age. Mycoplasma could be detected from tracheal swabs in this broiler breeder flock at age 49 weeks by culture and polymerase chain reaction (PCR) .Isolation and characterization of the recovered isolates using culture, biochemical, serological and PCR teste proved to be MG. Dot ELISA was applied in the current study and it was potential enough in rapid confirmation of Mycoplasma isolates. Histopathological examination of the trachea and lungs of infected broiler breeder flock. revealed the destructive effect of MG.

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