Evaluation of carotenoids produced by Rhodotorula glutinis using bioreactor with different feeding techniques on albino rats [electronic resource].
Language: English Summary language: Arabic Description: P.61-73Other title:- تقييم الكاروتينات المنتجة بواسطة رودوتوريولا جلوتينس بإستخدام المخمر بطرق تغذية مختلفة على الفئران البيضاء [Added title page title]
- Annals of agricultural science, 2007 v. 52 (1) [electronic resource].
Includes reference.
Carotenoids production was studied by cultivating R. glutinis 32 on modified MM-4 in bioreactor as a batch, two stage batch and fed batch cultures. Five incubated temperatures as well as five agitation speeds were tested for carotenoids production using batch bioreactor technique. The highest figures of carotenoids concentration and productivity were 4.24 mg L-', and 0.044 mg L-'h-' respectively on MM-4 incubated at 32C, 550 rpm after 72 h using two stage batch technique, while 44% and 27% were loss respectively on molasses medium. Two feeding rates of glycerol were studied during the production of carotenoids using fed batch culture, the highest carotenoids parameters were recorded at 4.0 gL-' h-' glycerol feeding rate. The latter technique increased the concentration, content and productivity of carotenoids by R. glutinis to 1.26, 1.09 and 1.26 fold respectively as compared with that obtained in batch bioreactor technique after 72 hr. The statistical analysis of animal feeding on plant, microbial carotenoids, or normal diet proved that the difference in T3, T4 hormones and daily gain among the three groups were insignificant. GOT and ALP enzymes as well as urea concentration in blood had no significant differences, except GPT and uric acid concentration among the three groups. The histopathological studies showed normal histological structure with no clinical carcino- genic signs either of liver or kidney rats of the three groups.
Summary in Arabic.
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