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Preparation of elisa kit for detection of antibodies to bovine herpes virus 1 [electronic resource].

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Khatfab, O.M

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Description: P.611-621Uniform titles:

Veterinary medical journal, 2007 v.55 (2) [electronic resource].

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In: Veterinary Medical Journal 2007.v.55(2)Summary: In .the present study, an ELISA assay was developed for detection of antibodies against BHV- 1. Colorado strain of BHV-I was propagated on MDBK cell and characterized by using SNT and FAT. The virus was titrated after and before concentration by polyethylene glycol (PEG). The protein content of the concentrated virus was determined. In the optimization steps of ELISA kit, the best results were at antigen concentration of 51Jg/mi and 1/25 dilution of serum. The efficiency of the prepared ELISAwas studied by comparison the results with those of SNT when applied both tests on 86 serum samples. 85 % of these samples were• positive by ELISA and only 61% were positive by SNT.

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In .the present study, an ELISA assay was developed for detection of antibodies against BHV- 1. Colorado strain of BHV-I was propagated on MDBK cell and characterized by using SNT and FAT. The virus was titrated after and before concentration by polyethylene glycol (PEG). The protein content of the concentrated virus was determined. In the optimization steps of ELISA kit, the best results were at antigen concentration of 51Jg/mi and 1/25 dilution of serum. The efficiency of the prepared ELISAwas studied by comparison the results with those of SNT when applied both tests on 86 serum samples. 85 % of these samples were• positive by ELISA and only 61% were positive by SNT.

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