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Fertilization capacity of rooster spermatozoa upon the biochemical changes of semen [electronic resource].

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El-Gendy, E. A

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Language: English Summary language: Arabic Description: p.406-411Other title:

كفاءة الأخصاب للحيوانات المنوية للديوك عند تغيير التركيب الكيميائي الحيوي للسائل المنوى [Added title page title]

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Bulletin of Faculty of Agriculture. Cairo University, 2012 v. 63 (4) [electronic resource].

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In: The Bulletin Faculty of Agriculture, Cairo University 2012.v.63(4)Summary: An experiment was carried out to study the effects of biochemical changes in rooster semen on the fertilization capacity of the spermatozoa. Chickens of two lines (CE2 and CE4) were used. Seven treatments of semen were designed and included the incubation of sperm with a bacterial plasmid (PUK18), a mixture of the plasmid and lipofectin at 5 or 2.5% concentration and the incubation of spermatozoa with lipofectin and a semen extender (BPSE). The progenies were obtained from the insemination of hens by the semen of different treatments. Sperm motility was greatly influenced by the treatments. Motility was significantly the highest in the control semen and averaged 92.42% and highly significantly declined to the renege of52.08 and 58.75% in the semen samples treated with the plasmid, Iipofectin at 2.5 or 5% concentration and diluted with BPSE. The percentage of live sperm was not affected by the addition of the plasmid.

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An experiment was carried out to study the effects of biochemical changes in rooster semen on the fertilization capacity of the spermatozoa. Chickens of two lines (CE2 and CE4) were used. Seven treatments of semen were designed and included the incubation of sperm with a bacterial plasmid (PUK18), a mixture of the plasmid and lipofectin at 5 or 2.5% concentration and the incubation of spermatozoa with lipofectin and a semen extender (BPSE). The progenies were obtained from the insemination of hens by the semen of different treatments. Sperm motility was greatly influenced by the treatments. Motility was significantly the highest in the control semen and averaged 92.42% and highly significantly declined to the renege of52.08 and 58.75% in the semen samples treated with the plasmid, Iipofectin at 2.5 or 5% concentration and diluted with BPSE. The percentage of live sperm was not affected by the addition of the plasmid.

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