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Physical mapping of knob-related sequences in some maize determined by FISH (fluorescent in situ hybridization) and fiber-FISH [electronic resource].

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Adawy, Sami S

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Language: English Summary language: Arabic Description: p.237-248Other title:

الخريطة الفيزيقية للـKnob فى بعض سلالات الذرة باستخدام تقنية التهجين الفلورسنتى FISH والتهجين الفلورسنتى لخيط واحد من الكروماتين fiber-FISH [Added title page title]

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Arab journal of biotechnology, 2002 v. 5 (2) [electronic resource]:

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In: Arab Journal of Biotechnology 2002.v.5(2)Summary: In situ hybridization (ISH) was performed simultaneously to localize and determine the linkage of two labeled probes (TRI and NOR) on the C-metaphase chromosomes of 18 inbred lines and varieties of maize (Zea mays L.). The tandemly repeated DNA sequences TRI (350-bp unit repeats) were labeled with Biotin, while the nucleolar organizer regions (NORs) were labeled with digoxigenin. Analysis of 10 Egyptian lines (Inb2, Inb6, Sd7, Inb8, Sd34, Inb37, Sd58, Inb60, Sd62 and Inb72) and 8 American inbreds and varieties (C103, MO17, Parkers flint E742, Parker flint G7390, Tamaflint, Wilbursflint, N/OI8 and DK493). Revealed that the two markers are located on different chromosomes and each of them occupied only one locus in all genotypes. Moreover, extended DNA-fibers (EDFs)-FISH was used to estimate and compare the physical length of the 350 bp repeat locus in the genome of three Egyptian maize inbred lines (Inb2, Sd58, and Sd62) quantitatively. The results revealed that the physical distances of the 350 bp repeat were 939.3, 960.4 and 912.4 Kb, respectively. Keywords: Fluorescence in situ hybridization, NOR, TR-1 (350bp), repetitive DNA sequences, C-metaphase, extended DNA fiber-FISH.

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In situ hybridization (ISH) was performed simultaneously to localize and determine the linkage of two labeled probes (TRI and NOR) on the C-metaphase chromosomes of 18 inbred lines and varieties of maize (Zea mays L.). The tandemly repeated DNA sequences TRI (350-bp unit repeats) were labeled with Biotin, while the nucleolar organizer regions (NORs) were labeled with digoxigenin. Analysis of 10 Egyptian lines (Inb2, Inb6, Sd7, Inb8, Sd34, Inb37, Sd58, Inb60, Sd62 and Inb72) and 8 American inbreds and varieties (C103, MO17, Parkers flint E742, Parker flint G7390, Tamaflint, Wilbursflint, N/OI8 and DK493). Revealed that the two markers are located on different chromosomes and each of them occupied only one locus in all genotypes. Moreover, extended DNA-fibers (EDFs)-FISH was used to estimate and compare the physical length of the 350 bp repeat locus in the genome of three Egyptian maize inbred lines (Inb2, Sd58, and Sd62) quantitatively. The results revealed that the physical distances of the 350 bp repeat were 939.3, 960.4 and 912.4 Kb, respectively. Keywords: Fluorescence in situ hybridization, NOR, TR-1 (350bp), repetitive DNA sequences, C-metaphase, extended DNA fiber-FISH.

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