Effect of ovary preservation period on recovery rate and categories of dromedary camel oocytes [electronic resource].

Includes references.

The aim of this study was to evaluate the effectof different periods of ovary preservation at 25-30 °c for 5, 6, 7, g, 12 and 24 hours on recovery rate and oocyte categories of dromedary camel oocytes. Camel ovaries were. collected from EIBassatein slaughterhouse, Cairo. The collected ovaries were placed immediately after slaughtering into thermos in saline solution (O.g% NaCI) supplemented with antibiotics (100 IU penicillin and 100ug streptomycin/mil at 25-30'C and transported to the laboratory within 4-5h. Ovaries were washed three times with warmed (30·C) phosphate buffer solution (PBS) and one time with ethanol (70%). All visible follicles on the ovarian surface (2-8 mm in diameter) were counted. Oocytes were aspirated using a 20-gauge hypodermic needle. Oocyte yield was recorded and the number of oocytes/ovary was calculated. Oocytes were classified into five categories (compactpartial denuded - denuded - shrunken and cleaved oocytes). Results show that average number of follicles on each ovary was not significantly affected by preservation period, although tended to reduced only after 5 hours of ovary preservation.

Summary in Arabic.

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