Molecular characterization of Egyptian watermelon promising lines for resistance to fusarium wilt [electronic resource].

Includes references.

Sequence characterized amplified region (SCAR) and random amplified polymorphic DNA (RAPD) were used to identify new watermelon promising lines for Fusarium wilt resistance. Six promising lines namely, 74, 6311, 95, 85, 84, 84g compared with Sugar Baby cultivars as a susceptible genotype were used in this study. SCAR, related to resistance watermelon with 898 bp distinguished the resistant lines which was consistent with the field reaction RAPD markers were used for characterization of the promising lines, three additional bands were provided related to primers 2, 5 and 6. Based on phylogenetic tree, there were two clusters, the first one consisted of two genotypes Sugar Baby and line 6311 as susceptible genotypes. While, the second consisted of two subclusters at the level of 80% genetic similarity, one of them has the three resistant genotypes, lines 74, 84 and 84 g. The second subcluster consisted of two lines 85 and 95 which were pre-identified as resistant lines. Thus, these markers could be useful for identifying Fusarium wilt-resistant genotypes in breeding programs using segregating progenies of watermelon.

Summary in Arabic.

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