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Phenotypic and genotypic identification of flouroquinolones resistant Escherichia coli isolated from diseased chicken in Suez Canal district [electronic resource].

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El-Shazly, Dalia A

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Language: English Summary language: Arabic Description: p.1-24Other title:

التعريف الظاهري والجيني للميكروب القولوني المقاوم للفلوروكينولون المعزول من الدجاج المصاب في منطقة قناة السويس [Added title page title]

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kafrelsheikh veterinary medical journal, 2014 v. 12 (1) [electronic resource].

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In: Kafrelsheikh Veterinary Medical Journal 2014.v.12(1)Summary: A total of 70 Escherichia coli strains were isolated from samples (liver and heart) collected from diseased chickens (n= 105) in about 21 commercial farms located in Suez Canal district of Egypt during May-July 2012. We investigated the susceptibility to Flouroquinolones (Ciprofloxacin, Enrofloxacin and Norfloxacin) and 9 other antibacterials (Gentamicin, Streptomycin, Doxycyclin, Amoxicillin, Ampicillin, Cephazolin, Cephradine, Lincomycin, Florfenicole) by disc diffusion method and measured the MIC of ciprofloxacin and enrofloxacin. Resulted flouroquinolones resistant E. coli isolates were then serotyped, and investigated for the prevalence of plasmid- mediated fluoroquinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA) by polymerase chain reaction. Among the E.coli isolates, 72.8% (51/70) were resistant to Ciprofloxacin and Enrojloxacin with MIC ≥ 4 and ≥ 2 (µg/ml) respectively.

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A total of 70 Escherichia coli strains were isolated from samples (liver and heart) collected from diseased chickens (n= 105) in about 21 commercial farms located in Suez Canal district of Egypt during May-July 2012. We investigated the susceptibility to Flouroquinolones (Ciprofloxacin, Enrofloxacin and Norfloxacin) and 9 other antibacterials (Gentamicin, Streptomycin, Doxycyclin, Amoxicillin, Ampicillin, Cephazolin, Cephradine, Lincomycin, Florfenicole) by disc diffusion method and measured the MIC of ciprofloxacin and enrofloxacin. Resulted flouroquinolones resistant E. coli isolates were then serotyped, and investigated for the prevalence of plasmid- mediated fluoroquinolone resistance (PMQR) genes (qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA) by polymerase chain reaction. Among the E.coli isolates, 72.8% (51/70) were resistant to Ciprofloxacin and Enrojloxacin with MIC ≥ 4 and ≥ 2 (µg/ml) respectively.

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