Polymerase chain reaction as diagnostic method for helicobacter pylori in comparison with other conventional methods [electronic resource].

By: Contributor(s): Language: English Summary language: Arabic Description: P. 85-91Other title:
  • تفاعل انزيم عديد البلمرة كطريقة تشخيصيه لحلزونيات البيلوري بالمقارنة بالطرق الاخري التقليدية [Added title page title]
Uniform titles:
  • Zagazig veterinary journal, 2008 v. 36 (1) [electronic resource].
Subject(s): Online resources: In: Zagazig Veterinary Journal 2008.v.36(1)Summary: Helicobacter pylori is one of the most common infections world-wide. The present study was designed to isolate the H. pylori from human, canine and feline. Identification of the isolated strain by studying their culture characters and biochemical activities. Performing RAPD PCR were done between the isolates of animal origin (canine and feline and the isolates of human origin) to detected any similarity or differences between them. Computer analysis for data was done. The obtained results revealed that 28 isolated from feline, 28 isolates from canine and 25 isolates from human was identified by biochemical activities, ELISA and genetic distances, calculated as the total number of band differences, between H. pylori strains, is (3.606) H. pylori strain isolated from canine and H. pylori isolated from feline and 3.876 between feline and human and 4.690 between H. pylori isolated from canine and human.
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Helicobacter pylori is one of the most common infections world-wide. The present study was designed to isolate the H. pylori from human, canine and feline. Identification of the isolated strain by studying their culture characters and biochemical activities. Performing RAPD PCR were done between the isolates of animal origin (canine and feline and the isolates of human origin) to detected any similarity or differences between them. Computer analysis for data was done. The obtained results revealed that 28 isolated from feline, 28 isolates from canine and 25 isolates from human was identified by biochemical activities, ELISA and genetic distances, calculated as the total number of band differences, between H. pylori strains, is (3.606) H. pylori strain isolated from canine and H. pylori isolated from feline and 3.876 between feline and human and 4.690 between H. pylori isolated from canine and human.

Summary in Arabic.

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