Production of some new peach rootstock through hybridization and tissue culture technique [electronic resource].

Includes references.

This study was carried out through three succeeded years (2005- 2007) in almond- peach orchard aimed to grow plantlets of new hybrids coming from hybridization between both of Om El-fahm X Hansen by using tissue culture technique and to determine some molecular markers related to root -knot nematode resistance were detected. In field experiment, height of hybrids seedlings ranged from 53.3-70.1 cm, 2.21- 2.65 cm for stem thickness and 41 -63 for leaves number per one. As for tissue cu lture experiment, stem node was superior than shoot tip on bud development and shoot length in establishment stage. Meanwhile, th e third subculture records the greatest significant number of produced shoots and shoot length in multiplication stage. for rooting stage, all genotype hybrids raised mediated ranged from 53.3-73.3 &4.16-5.33 and 3.14-5.11 for rooting %, root number/ shoot and root le ngth, respectively. Hybrid genotype data confirmed that hybrid no.4 wa s superior than the other hybrids. By using four primers pairs relate d to root knot nematode resistance by two techniques. Pchgms primer by SSR and OPA11, OP834B and OPAP4 by STS were used. Four molecular markers appeared in hybrid no. 4 only which may be the mo st hybrid can be resistant to This study was carried out through three succeeded years (2005- 2007) in almond- peach orchard aimed to grow plantlets of new hybrids coming from hybridization between both of Om El-fahm X Hansen by using tissue culture technique and to determine some molecular markers related to root -knot nematode resistance were detected. In field experiment, height of hybrids seedlings ranged from 53.3-70.1 cm, 2.21- 2.65 cm for stem thickness and 41 -63 for leaves number per one. As for tissue cu lture experiment, stem node was superior than shoot tip on bud development and shoot length in establishment stage. Meanwhile, th e third subculture records the greatest significant number of produced shoots and shoot length in multiplication stage. for rooting stage, all genotype hybrids raised mediated ranged from 53.3-73.3 &4.16-5.33 and 3.14-5.11 for rooting %, root number/ shoot and root le ngth, respectively. Hybrid genotype data confirmed that hybrid no.4 wa s superior than the other hybrids. By using four primers pairs related to root knot nematode resistance

Summary in Arabic.

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