The association of botanical traits with molecular markers using induced mutants of faba bean [electronic resource].

Includes references.

Twenty-four faba bean mutants were selected and treated with gamma rays doses of 30 and 60 Gy and ethyl methane sulfonate (EMS)at conc.of 0.15 and 0.30%. The effect of mutagens was studied using a number of parameters,such as morphological growth characters, physiological parameters, flowering, yield attributes and the genetic characterization of all protein and DNA molecular markers based on SDS-PAGE RAPD analysis. The data showed that the induced mutants by either gamma rays or EMS tended to have higher values in most growth characters and the maximum increase was achieved by the mutant lines No.7, 14, 24, 25, 18, 19 and 26. Mutant lines which were produced by (EMS) were more effective in increasing the concentrations of photosynthetic pigments, ChI. b, carotenoids and total ch!orophylls than that produced by gamma ray. Transpiration rate (TR) exceeded the control in all mutants produced by gamma radiation except for mutant line No. 18. On the other hand, mutants No.5, 12, 20, 23, 24 and 25 which were produced by EMS also exceeded the control. Mutant lines produced _ by gamma rays were more effective than that produced by (EMS). As for shelling percentages, the mutant lines No.7, 8, 9, 21, 23 and 18 showed a significant increase while the mutant lines No.1, 4, 19, and 26 showed considerable lower values than the control. The increase in yield and yield components using physical and chemical mutagens was noticed. It was possible to associate the specific polymorphic DNA fragments of about 3000 bp and 200 bp in size which were generated by the random primers (A1, A5 and A3) for Gy or EMS mutants markers might be of great importance in future programs of Vicia faba plants breeding and selection for mutants, with both high yield and early flowering.

Summary in Arabic.

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