Identification of quantitatve trait loci associated with leaf rust resistance in wheat (Triticum aestivum L ) using microsatellite markers.

By: Contributor(s): Language: English Summary language: Arabic Description: p.76-87Other title:
  • تحديد مواقع الصفات الكمية لمقاومة صدأ الورقة في القمح باستخدام الميكروساتيلايت [Added title page title]
Uniform titles:
  • Journal of the advances in agricultural researches, 2013 v. 18 (1) [electronic resource].
Subject(s): Online resources: In: Journal of the Advances in Agricultural Researches 2013.v.18(1)Summary: This study is conducted to identify quantitative trait loci (QTLs) for leaf rust reSistance in F2 population of wheat by using bulked segregant analySis (BSA) with simple sequence repeat (SSR) markers. F2 population were resulted from a cross between the variant line (S11), which is susceptible to leaf rust and the resistant (CIMMYT) variety Pavon 76. F2 of 101 individuals along with their parental lines were analyzed with thirty three pairs of simple sequence repeats (SSRs) markers. The results showed that only two of SSR primer pairs (Xgwm 428 and Xgwm334) gave polymorphic DNA fragments. A simple single marker regression analysis showed that these two markers were found to be linked to leaf rust QTL (LrD and Lr A). R2 explained by markers Xgwm 428 and Xgwm 334 were 43 and 55%, due to the segregation of the QTL respectively. The LrD and LrA genes, mapped on chromosome 7D and 6A respectively, may act in a complementary interaction.
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This study is conducted to identify quantitative trait loci (QTLs) for leaf rust reSistance in F2 population of wheat by using bulked segregant analySis (BSA) with simple sequence repeat (SSR) markers. F2 population were resulted from a cross between the variant line (S11), which is susceptible to leaf rust and the resistant (CIMMYT) variety Pavon 76. F2 of 101 individuals along with their parental lines were analyzed with thirty three pairs of simple sequence repeats (SSRs) markers. The results showed that only two of SSR primer pairs (Xgwm 428 and Xgwm334) gave polymorphic DNA fragments. A simple single marker regression analysis showed that these two markers were found to be linked to leaf rust QTL (LrD and Lr A). R2 explained by markers Xgwm 428 and Xgwm 334 were 43 and 55%, due to the segregation of the QTL respectively. The LrD and LrA genes, mapped on chromosome 7D and 6A respectively, may act in a complementary interaction.

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