Application of protein related index and isozymes in defining ziccini yellow mosaic potyvirus in infected squash plants [electronic resource].

Includes reference.

Squash plants naturally infected by Ziccini yellow mosaic potyvirus (ZYMV) were classified into three groups according to the degree of symptoms i.e. mosaic, curly and crinkling, and malformation leaves. The disease was transferred from each group to various cucurbaceae plants by sap mechanical technique then diagnosed by biological and serological (DAS-ELISA) tests, and change in chlorophyll contents to ensure that all these symptoms resulted from ZYMV. The use of protein related index (PR-index) and isozyme separation to detect ZYMV infection in squash plants and to distinguish each degree of symptom was evaluated. SDS-PAGE of protein separation showed a distinct protein pattern and protein contents of control and infected plants. DISC-PAGE isozyme separation of control and the three infected groups showed 7, 6, 6 and 8 esterase isozymes, and 4, 6, 2 and 7 peroxidase isozymes respectively. Similarity analysis confirmed the difference in protein and isozyme patterns between control and each of infected plant groups, which suggests using these techniques in ZYMV characterization. Key words: Soluble proteins, Peroxidase and esterase isozymes, ZYMV, Biological and serological assays, Photopegment, Squash plants.

Summary in Arabic.

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