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Genetic diversity among four sheep crossbreds based on detected random amplified polymorpidc DNA markers [ electronic resource].

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Mahmoud, A.A

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Language: English Summary language: Arabic Description: p.685-701Other title:

التباين الوراثى بين أربعة من هجن الأغنام باستخدام واسمات الرابيدRAPD [Added title page title]

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Zagazig journal of agricultural research, 2007 v. 34 (4) [electronic resource].

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In: Zagazig Journal of Agricultural Research 2007.v.34(4)Summary: Blood samples were collected via jugular vein from four crossbreds of sheep, CBI, CB2, CBJ and CB4. Random amplified polymorphic DNA analysis was used to fingerprint these crossbreds in order to study the genetic relationships between them and to search for genetic markers in each one. In this respect, many random primers were applied, but only seven primers (A02, A04, A07, A09, AIO, A12, and AI7) were succeeded in the amplification of genomic DNA from these sheep breeds. Several positive and negative specific bands (markers) which are differentiated between crossbreds were detected by five primers. Primer A02 was the best one, exhibiting markers among the four crossbreds followed by both A04 and A09 primers, which gave markers for three crossbreds, while A12 revealed specific markers for two crossbreds and AI7 for only one crossbred. On the other hand, neither A07 nor AIO primers gave any specific band.

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Blood samples were collected via jugular vein from four crossbreds of sheep, CBI, CB2, CBJ and CB4. Random amplified polymorphic DNA analysis was used to fingerprint these crossbreds in order to study the genetic relationships between them and to search for genetic markers in each one. In this respect, many random primers were applied, but only seven primers (A02, A04, A07, A09, AIO, A12, and AI7) were succeeded in the amplification of genomic DNA from these sheep breeds. Several positive and negative specific bands (markers) which are differentiated between crossbreds were detected by five primers. Primer A02 was the best one, exhibiting markers among the four crossbreds followed by both A04 and A09 primers, which gave markers for three crossbreds, while A12 revealed specific markers for two crossbreds and AI7 for only one crossbred. On the other hand, neither A07 nor AIO primers gave any specific band.

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