Mannheimia haemolytica and Pasteurella multocida pneumonia in sheep and goats in Fayoum Governorate [electronic resource]: the use of ELISA technique.

Includes references.

A total of 299 cases of small ruminant animals (193 sheep and 106 goats) from different localities of Fayoum governorate were subjected to clinical examination for respiratory manifestations! Nasopharyngeal swabs and blood samples were separately collected from all studied animals. Also, a total of 138 lung tissues samples were collected from 94 sheep and 44 goats from different abattoirs. These samples consisted of 68 and 32 lungs from sheep and goats respectively showed pneumonia manifestation. The rest samples (26 and 12 respectively) were from normal healthy lungs. Swabs and lung samples were subjected for bacteriological examination and The Enzyme linked immunosorbeot assay (ELISA) test was carried out on sera samples. Bacteriological examination revealed the isolation of M. haemolyticq and P. multocida either in pure culture or mixed with other different bacteria such as E. coli, Kl. pneumonae, P. aerogenosa and S. aureus. M haemolytica and P. mltocida was recovered in a percentage of20.6 % and 21.3 % from studied sheep and goats respectively. The recoveIY- rate from diseased cases was significantly higher than the apparent healthy cases, almost double the incidence. The M. haemolytiea capsular A and T antigens, and the somatic antigens type A: 2 T: 4; T: 10 and T: 15 were detected with percentages of 58.3,41.7,52.8,25.0, 16.7 and 5.5 respectively. The highest incidence was recorded with capsular antigen A (58.3 %) and somatic antigen type A: 2 (52.8 %) in both sheep and goats. Pasteurella multoeida isolates showed capsular antigen type A (70.9%) and type D (21.8%), but 4 isolates (7.3%) were untyped. The somatic antigen of the typed strain's were identified as A:2, A:5, D:1, D:3 and D:4 with a percentage of 49.0, 17.7, 19.6, 9.5 ,and 3.9 respectively. The highest incidence was reported with capsular antigen A (70.9 %) fUld somatic antigen rype A: 2 (49.0 8%) in both sheep and goats. It is worthy to denote that the 2 strains identified as D: 4 were goat's isolates. From our results, it could be concl~ded that identical Pasteurella strains were shared by the goats and sheep. The recovery rate for ELISA was found to be 95.5 % and 94.7% for M. haemolytiea and that Pasteurella multoeida respectively, which considered highly significant. The ELISA technique as a "culture-independent methods" was proved to be easy, practical, sensitive and efficient method for quick diagnosis of M. haemolytiea and Pasteurella multocida infection in sheep and goats.

Summary in Arabic.

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