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Comparison of RIDA®quick verotoxin/O157 test, verocytotoxicty assay and PCR in detection of shiga toxins in cultures of human stool, animal faeces and meat products [electronic resource].

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Zidan, S. A

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Language: engbara Description: p.14-22Other title:

مقارنة استخدام بعض الاختبارات الحديثة فى الكشف عن الميكروب القولونى المفرزلتوكسين شيجا فى الحيوانات والانسان ومنتجات اللحوم.‪ [Added title page title]

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Assiut veterinary medical journal, 2014 v. 60 (142) [electronic resource].

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In: Assiut Veterinary Medical Journal 2014.v.60(142)Summary: Shiga toxins (Stx) are the major virulence factors of STEC, which cause diarrhea, life-threatening haemolytic uraemic syndrome (HUS) and acute renal failure in children. This work aimed to evaluate the new RIDA®QUICK Verotoxin/Ol57(R- Biopharm,Darmstadt, Germany), which detects Stx & E. coli serogroup 0157 and to determine the extent of faecal contamination of meat products by STEC. A total 623 samples, including 214 faecal samples collected from sheep, 209 stool samples from human and 200 samples of different meat products were cultured on Trypticase Soya Broth and CT -Sorbitol MacConkey agar, samples were Biochemically identified as Escherichia coli. Further identifications were performed by serotyping and Vero cells cytotoxicity assay. PCR for specific VT1/VT2 genes and RIDA ®QUICK Verotoxin/0157 were applied on VCA positive samples. The results of RIDA®QUICK revealed the detection of STEC in 14(93.33%), 6 (85.71%) and 13(86.66%) while PCR test detected 15(100%), 7(100%) and 15(100%) VCA positive samples from sheep, man and meat products, respectively.

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Shiga toxins (Stx) are the major virulence factors of STEC, which cause diarrhea, life-threatening haemolytic uraemic syndrome (HUS) and acute renal failure in children. This work aimed to evaluate the new RIDA®QUICK Verotoxin/Ol57(R- Biopharm,Darmstadt, Germany), which detects Stx & E. coli serogroup 0157 and to determine the extent of faecal contamination of meat products by STEC. A total 623 samples, including 214 faecal samples collected from sheep, 209 stool samples from human and 200 samples of different meat products were cultured on Trypticase Soya Broth and CT -Sorbitol MacConkey agar, samples were Biochemically identified as Escherichia coli. Further identifications were performed by serotyping and Vero cells cytotoxicity assay. PCR for specific VT1/VT2 genes and RIDA ®QUICK Verotoxin/0157 were applied on VCA positive samples. The results of RIDA®QUICK revealed the detection of STEC in 14(93.33%), 6 (85.71%) and 13(86.66%) while PCR test detected 15(100%), 7(100%) and 15(100%) VCA positive samples from sheep, man and meat products, respectively.

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