Prevalence and antimicrobial susceptibility of Staphylococcus aureus isolated from cattle, buffalo, sheep and goat's raws milk in Sohag governorate, Egypt [electronic resource].

Includes references.

The objective of this study is to determine the prevalence rate of S. aureus in cow, buffalo, sheep and goat's raw milk in Sohag Governorate, Egypt by using conventional methods and molecular technique and further characterization of their antimicrobial resistance. Isolation and identification of S. aureus were performed for a totally of 340 raw milk samples from cow, buffalo, sheep and goat's milk randomly selected from dairy farms and shops. Altogether, S. aureus was isolated from 136 raw milk samples (40%) of the 340 milk samples 44(36.7%) from fresh cow milk samples 65(46.4%) from buffalo milk 12(30%) from sheep milk and 15(37.5%) from goat's milk samples. Amplification of genes encoding clumping factor (clfA) and thermonuclease (nuc) gene by Polymerase Chain Reaction was used for the genotypic characterization of isolated S. aureus strains. All tested S. aureus strains yielded a single amplicon with a size of approximately 985 bp by amplification of the (clfA) gene, however, amplification of the ( nuc) gene produced an amplicon of 270 bp in all examined S. aureus isolates. The susceptibilities of the isolates were determined for 11 antimicrobial drugs using the disk diffusion assay.

Summary in Arabic.