Partial nucleotide sequence of segment one encoding replicase gene of banana bunchy top nanovirus [electronic resource].

Includes references.

This study was designed to partially determine the nucleotide sequences of banana bunchy top nanovirus (BBTV) DNA-1 followed by sequences analysis to compare some overseas BBTV isolates. This was carried out by confirmation of the presence of BBTV in diseased banana plants collected from El-Kalubia Gover-norate in Egypt using monoclonal anti-bodies (MAbs) specific to the Australian BBTV isolate. The rep fragment was amplified by using polymerase chain reaction (PCR), cloned into pGEM-T easy vector and sequenced. Results showed that a fragment of about 603 belonging to the replicase gene of BBTV was sequenced and a number of 201 amino acids (a.a) were deduced. The identity of the rep gene of this study was determined comparing with seven BBTV isolates from India and one isolate from the following countries: Australia, Viet Nam, Taiwan, China and Egypt that were published in Gene Bank. The relation was illustrated by phylogenetic trees. Isolate of this study showed the highest identities with that of Maharastra (India) as 99 and 100 % similarities between DNA and a.a sequences, respectively were found. The 32P-DNA-PCR labeled probe amplified from BBTV-infected plants was successfully used to differentiate between recombinant and non-recombinant clones in Southern analysis of BBTV.

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