Some factors affecting micropropagation of some wild medicinal plants [electronic resource]: 1-Ecballium elaterium L. plant.

Includes reference.

Excised embryos of Ecballium elaterium L. (squirting cucumber, wild cucumber) was cultured on MS medium supplemented with 2, 4-D at 0, 1 or 2 mg/l and kinetin at 0, 1 or 2 mg/l. Kinetin alone did not induce callus formation. Using 2, 4-D at 1 or 2 mg/l alone show the less response of callus fresh weight while the ejective combinations of 2, 4¬D and kinetin were 1 mg/l 2, 4-D combined with 1 mg/l kinetin or 2 mg/l 2, 4-D combined with 2 mg/l kinetin which resulted in the highest fresh weight of callus after 30 and 60 days from embryos culture without significant differences between the two treatments. Similar excised pieces of callus cultured on MS medium supplemented with combinations of benzyl adenine (BA) at 0.5 or 1.0 or 1.5 mg/l and naphthalene acetic acid (NAA) at 0.1, 0.2 or 0.3 mg/l showed the less differentiations of shoot number with NAA alone, but the best combination which resulted in the highest shoots number was 0.5 mg/l BA combined with 0.1 mg/l NAA or 1.5 mg/l BA combined with 0.2 mg/l NAA. Shoot length resulted in the highest value with the later combination. Where, the highest number of leaves/ shoot was obtained from 0.5 mg/l BA combined with 0.1, 0.2 or 0.3 mg/l NAA. The excised shoots showed the highest number of roots and root length on //4 !viS medium strength supplemented with 0.3 mg/l indole-3-butyric acid (IBA). The obtained plantlets were successfully acclimatized on peat moss: sand (I: I) medium with 90% survival plants.

Summary in Arabic.

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