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Detection of Ralstonia solanacearum on some crops and weeds under Egyptian conditions [electronic resource].

By:

Hamad, Y.I

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Language: English Summary language: Arabic Description: p.769-788Other title:

الكشف عن بكتريا راستونيا سولاناسيرم على بعض المحاصيل والحشائش تحت الظروف المصرية [Added title page title]

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Zagazig journal of agricultural research, 2008 v. 35 (4) [electronic resource].

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In: Zagazig Journal of Agricultural Research 2008.v.35(4)Summary: The present investigation was carried out to identify weed and crop hosts of Ralstonia solanacearum Yabuuchi et al., the causal agent of potato brown rot, under EI-Sharkia and EI-Ismailia Egyptian governorates. Obtained results revealed that weed species Solanum nigrum L. (black nightshad), Portulaca oleracea L. (purslane), Brassica kaber (DC) L.C. (black mustard), Chenopodium album L. (lamb's paurters, Beta vulgaris L. (curly dock), Rumex dentatus L. (sorrel) are considered as hosts for R. solanacearum. On the other hand, Amaranthus sylvestris L. (pig weed), Cichorium I endivia L. (wild chicory), Conyza discorids L., Conyza lin/olia (wild.) Tackhi, Sonchus oleraceus L., Convolvulus arvensisn L. (field bind), Sisymbrium irio L., Medicago capulina L., Plantago major L. (great plantain) and Urtica urens L. (small nettle) not considered hosts for R. solanacearum. Twelve isolates of R. solanacearum were isolated from weeds species. Isolates R. solanacearum (race 3 biovar 2) were identified using morphological, physiological and biochemical tests. Also advanced Immunofluorescence Antibody Stain (IFAs) and Polymerase Chain Reaction (PCR) techniques were applied and confirmed that the isolates are related to R. solanacearum.

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The present investigation was carried out to identify weed and crop hosts of Ralstonia solanacearum Yabuuchi et al., the causal agent of potato brown rot, under EI-Sharkia and EI-Ismailia Egyptian governorates. Obtained results revealed that weed species Solanum nigrum L. (black nightshad), Portulaca oleracea L. (purslane), Brassica kaber (DC) L.C. (black mustard), Chenopodium album L. (lamb's paurters, Beta vulgaris L. (curly dock), Rumex dentatus L. (sorrel) are considered as hosts for R. solanacearum. On the other hand, Amaranthus sylvestris L. (pig weed), Cichorium I endivia L. (wild chicory), Conyza discorids L., Conyza lin/olia (wild.) Tackhi, Sonchus oleraceus L., Convolvulus arvensisn L. (field bind), Sisymbrium irio L., Medicago capulina L., Plantago major L. (great plantain) and Urtica urens L. (small nettle) not considered hosts for R. solanacearum. Twelve isolates of R. solanacearum were isolated from weeds species. Isolates R. solanacearum (race 3 biovar 2) were identified using morphological, physiological and biochemical tests. Also advanced Immunofluorescence Antibody Stain (IFAs) and Polymerase Chain Reaction (PCR) techniques were applied and confirmed that the isolates are related to R. solanacearum.

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