Developmental competence of immature cytoplasts upon somatic nuclear transfer before and after maturation [electronic resource].

Includes reference.

The results indicated that survival rates of enucleation GV oocytes were similar with selective and complete enucleation techniques. Selective enucleation technique of GV oocytes resulted in obtaining pronuclei containing nucleoli after transfer of fetal fibroblast nuclei into the cytoplasts before/after maturation compared with complete enucleation which resulted in obtaining pronuclei without nucleoli. Reconstruction of cytoplasts after maturation resulted in the cleavage of activated oocytes compared with reconstruction of cytoplasts before maturation which resulted in uncleavage of activated oocytes. In conclusion, transfer of FFs into GVsel cytoplasts after maturation resulted in obtaining the best results.

Summary in Arabic.

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