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Effect of culture system on the survival of cryopreserved bovine embryos [electronic resource].

By:

Noseir, W.M.B

Language: English Summary language: Arabic Description: P.61-71Other title:

تأثير انظمة المنابت على بقاء اجنة الابقار المجمدة .‪ [Added title page title]

Uniform titles:

Veterinary medical journal , 2008 v.56 (1) [electronic resource].

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In: Veterinary Medical Journal 2008.v.56(1)Summary: This study compares the effect of culture system on the survival and hatching rates of in-vitro produced bovine embryos cryopreserved by slow freezing and vitrification. Oocytes were collected by slicing of ovaries from slaughtered cows. Presumptive zygotes produced by IVMIIVF were randomly allocated to one of the following culture media; TCM-l99 or SOP. Day 7 and day 8 blastocysts, cultured in TCM-l99 or SOF, were subjected to two cryopreservation methods; slow freezing and vitrification. Development of blastocysts 4, 24 and 48 hours post-thawing and hatching rates were recorded. There were no statistical differences in the post-thawing development or hatching rate between day 7 and day 8 cryopreserved blastocysts cultured in TCM-l99 or SOF.

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This study compares the effect of culture system on the survival and hatching rates of in-vitro produced bovine embryos cryopreserved by slow freezing and vitrification. Oocytes were collected by slicing of ovaries from slaughtered cows. Presumptive zygotes produced by IVMIIVF were randomly allocated to one of the following culture media; TCM-l99 or SOP. Day 7 and day 8 blastocysts, cultured in TCM-l99 or SOF, were subjected to two cryopreservation methods; slow freezing and vitrification. Development of blastocysts 4, 24 and 48 hours post-thawing and hatching rates were recorded. There were no statistical differences in the post-thawing development or hatching rate between day 7 and day 8 cryopreserved blastocysts cultured in TCM-l99 or SOF.

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